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. 2019 Mar 12;37(6):779–790. doi: 10.1002/stem.2996

Figure 5.

Figure 5

Stem cell‐derived extracellular vesicles (EVs) contain high levels of peroxiredoxins and reduce cellular reactive oxygen species (ROS) in senescent cells. (A): Peroxiredoxin abundance in induced pluripotent stem cell (iPSC)‐EVs and mesenchymal stem cell (MSC)‐EVs by proteomics, rank by iBAQ intensities in iPSC‐EVs. iBAQ, intensity based absolute quantification. (B): Western blotting to examine the protein level of PRDX1 and PRDX2 in plasma‐EVs, iPSC‐EVs and MSC‐EVs. (C): Western blotting analysis of PRDX1 and PRDX2 proteins in young MSCs (passage 3) and senescent MSCs (progerin‐induced). (D): Detection of cellular ROS by CellROX green in young MSCs (passage 3) and senescent MSCs (progerin‐induced). (E): AlamarBlue fluorometric assay to assess the cell growth of progerin‐induced senescent MSCs (control) compared with those treated with plasma‐EVs or iPSC‐EVs for 4 days. (F): Quantitation of PRDX1 and PRDX2 protein levels measured by Western blotting in cells cultured for 4 days with or without treatment with plasma‐EVs or iPSC‐EVs. (G): Detection of reduced cellular ROS level after treatment with iPSC‐EVs (red) versus untreated senescent MSCs (black). All data reflect mean ± SD from four independent experiments. *, p < .05; **, p < .01; ***, p < .001. Also see Supporting Information Figure S5 and Supporting Information Table S2.