Figure 2.

Anti‐fibrotic effect of butaprost is independent of cAMP/PKA signalling. MDCK cells were exposed to 5 ng/ml TGF‐β in the absence or presence of butaprost (50 μM), SQ22536 (75 µM) or H89 (10 µM) for 24 h. (A) cAMP levels were determined in cell lysates via ELISA (n = 6). (B, C) FN protein expression was studied using western blotting (n = 5‐6). Data are presented as mean ± SEM. *P < 0.05