Figure 5.

Butaprost mitigates fibrosis in UUO mice. Mice were subjected to 7 days of UUO and treated with butaprost (4 mg/kg). (A) FN and (B) αSMA protein expression was studied using Western blot (n = 6). (C‐E) Gene expression was studied by qPCR. Relative expression was calculated using the reference gene 18S (n = 6‐10). (F) Representative images of immunolabeling for αSMA (green) counterstained with DAPI (blue). 20× magnification, scale bar is 50 μm. (G) Hematoxylin and eosin staining of renal cortical tissue. 20× magnification, scale bar is 50 μm. Quantification of (H) interstitial and (I) tubular volume (n = 4). Data are presented as mean ± SEM. *P < 0.05