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. 2019 May 20;227(1):e13291. doi: 10.1111/apha.13291

Table 4.

Primer sequences

Target gene Accession number Forward Reverse
Murine
αSMA NM_007392.3 5′‐CTGACAGAGGCACCACTGAA‐3′ 5′‐CATCTCCAGAGTCCAGCACA‐3′
FN NM_010233.2 5′‐AATGGAAAAGGGGAATGGAC‐3′ 5′‐CTCGGTTGTCCTTCTTGCTC‐3′
EP2 NM_008964.4 5′‐ATGCTCCTGCTGCTTATCGT‐3′ 5′‐AGGGCCTCTTAGGCTACTGC‐3′
COL1A1 NM_007742.4 5′‐CACCCTCAAGAGCCTGAGTC‐3′ 5′‐ACTCTCCGCTCTTCCAGTCA‐3′
18S NM_011296.2 5′‐GAAAATAGCCTTCGCCATCA‐3′ 5′‐TCCCATCCTTCACATCCTTC‐3′
Human
EP2 NM_000956.3 5′‐CCACCTCATTCTCCTGGCTA‐3′ 5′‐TTCCTTTCGGGAAGAGGTTT‐3′
αSMA NM_001141945.2 5′‐ACCCACAATGTCCCCATCTA‐3′ 5′‐GAAGGAATAGCCACGCTCAG‐3′
FN NM_212482.2 5′‐CAGTGGGAGACCTCGAGAAG‐3′ 5′‐GTCCCTCGGAACATCAGAAA‐3′
COL1A1 NM_000088.3 5′‐CCTGGATGCCATCAAAGTCT‐3′ 5′‐AATCCATCGGTCATGCTCTC‐3′
RPL22 NM_000983.3 5′‐TCGCTCACCTCCCTTTCTAA‐3′ 5′‐TCACGGTGATCTTGCTCTTG‐3′
GAPDH NM_002046.5 5′‐ACCAGGGCTGCTTTTAACTCT‐3′ 5′‐GGTGCCATGGAATTTGCC‐3′
Canine
TGFβ NM_001003309.1 5′‐AAGAAAAGTCCGCACAGCAT‐3′ 5′‐GCTGCTCCGCTTTTAACTTG‐3′
GAPDH NM_001003142.2 5′‐AACATCATCCCTGCTTCCAC‐3′ 5′‐GGCAGGTCAGATCCACAACT‐3′