Figure 6.

miR‐582‐5P directly targeted Runx2. A schematic diagram illustrated the 3′UTR fragments of LRP, RICTOR, Runx2 (a). Transfection of miR‐582‐5p in 293T cells decreased the luciferase activity of Runx2 (d), while had no effects on LRP and RICTOR constructs (b, c). The miR‐582‐5p lost its inhibitory effect on the 3′UTR construct of Runx2 when its putative miR‐582‐5p binding site was mutated (e). All data are shown as mean ± SD. *p < 0.05, **p < 0.01. p Values were determined by the Student t test. LRP: low‐density lipoprotein receptor‐related protein; NC: negative control; RICTOR: rapamycin‐insensitive companion of mammalian target of rapamycin; UTR: untranslated region [Color figure can be viewed at wileyonlinelibrary.com]