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. 2019 Apr 29;86(7):762–775. doi: 10.1002/mrd.23153

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© 2019 The Authors. Molecular Reproduction and Development Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Effect of SPINK2 on serine proteases activity in F3 fraction eluted from benzamidine chromatography by reverse zymography. Gelatin zymograms of F3 fraction incubated with or without SPINK2 inhibitor reveal serine protease activity. Three major bands of proteolysis were identified at 38, 30, and 25 kDa corresponding to precursor and active forms of acrosin, respectively. The asterisk indicates areas where SPINK2 inhibits serine proteases activity. Lanes: 0, F3 fraction without SPINK2; 16, F3 fraction + 16 nM of SPINK2; 160, F3 fraction + 160 nM of SPINK2