Table 1.

Proteins identified by mass spectrometry in fractions after benzamidine chromatography with sperm

Description (Gallus gallus)	Gene symbol	Gene ID	Percent coverage (%)	MW (kDa)	Normalized emPAI
Dihydropyrimidinase	DPYS	420266	30.1	58	1.39
Acrosin‐a	ACR	426864	18.9	39	1.08
Acrosin‐b	ACR	769176	15.7	38	0.79
Sperm autoantigenic protein 17	SPA17	768714	17.4	16	0.47
1‐Phosphatidylinositol 4,5‐bisphosphate phosphodiesterase zeta‐1	PLCZ1	418182	18.1	73	0.36
Peptidylprolyl isomerase like 6	PPIL6	421764	19.0	33	0.33
Proteasome C1 subunit, partial	PSMB5	396003	13.7	22	0.33
Calcium binding tyrosine phosphorylation regulate	CABYR	107052002	13.6	35	0.31
Hexokinase 3	HK3	768421	7.43	110	0.26
Cluster of serum albumin precursor	ALB	396197	12.8	70	0.24
Neural EGF like 2	NELL2	417799	8.46	91	0.24
NME/NM23 family member 8	NME8	428461	6.74	66	0.16
PIT54 protein	PIT54	395364	7.45	51	0.13
Dynein axonemal intermediate chain 1	DNAI1	431654	5.82	81	0.13
Tektin‐5	TEKT5	416635	5.18	56	0.12
MYCBPAP associated protein	MYCBPAP	422101	4.07	109	0.092
Acyl‐CoA synthetase bubblegum family member 2	ACSBG2	420090	3.68	81	0.082
Adenylate kinase 7	AK7	428907	4.62	82	0.081
Dynein axonemal heavy chain 7	DNAH7	424048	3.77	459	0.073
Dynein axonemal heavy chain 3	DNAH3	427004	2.91	463	0.072
Cilia‐ and flagella‐associated protein 69 isoform X1	CFAP69	420542	2.68	105	0.063
Importin‐5	IPO5	418783	2.37	124	0.053
Dynein axonemal intermediate chain 10	DNAH10	416818	2.45	528	0.050
Androglobin	ADGB	421618	1.75	182	0.036
Cilia‐ and flagella‐associated protein 43 isoform X1	CFAP43	423879	2.24	194	0.034
Dynein axonemal intermediate chain 12	DNAH12	416004	1.46	453	0.029
Vacuolar protein sorting‐associated protein 13 C isoform X1	VPS13C	415376	0.56	422	0.015

Note. Proteases are shaded in gray.