Table 1.
Hydrotime analysis of the germination response to osmotic stress of mutant and complemented TIP3 and TIP4;1 lines
| Knockout/down and complemented lines | Mean base water potential (Ψb; MPa) | Standard deviation (σΨb) |
|---|---|---|
| Col‐0 (wild type) | −1.272* | 0.222 |
| tıp3;1 | −1.312** | 0.199 |
| tıp3;2 | −1.254 | 0.262 |
| tıp3;1 tıp3;2 | −1.175*, ** | 0.200 |
| YFP‐TIP3;2 | −1.260 | 0.171 |
| tıp4;1 | −1.326 | 0.228 |
| YFP‐TIP4;1 | −1.253 | 0.205 |
Note. Table shows the mean base water potential (Ψb) of the wild type (Col‐0) and all mutant and complemented lines. Ψb is the minimum water potential at which germination completion can occur. As Col‐0 is the parental line of all mutants and complemented lines, the Col‐0 hydrotime constant (θH) 34.352 MPa hr−1 was used as a standard for all lines in the analysis. Values followed by the same * or ** are significantly different (P < .05) from one another determined by comparisons between tip3 lines and Col‐0, and tip4 lines and Col‐0 using paired t tests assuming unequal variance.