Table 3.
Effects of loratadine (0.5–50 mg/kg p.o.) on histomorphometric parameters of the distal metaphysis, diaphysis, and distal epiphyseal cartilage in the femur in young male rats
| Parameter/group | C | L0.5 | L5 | L50 | |
|---|---|---|---|---|---|
| Metaphysis | BV/TV (%) | 37.4 (31.7–48.6) | 40.9 (32.1–47.2) | 38.0 (31.2–49.7) | 40.0 (28.5–54.7) |
| Tb.Th (μm) | 55.5 (52.1–78.8) | 62.9 (49.4–67.4) | 64.2 (54.5–72.0) | 59.1 (51.1–74.5) | |
| Tb.Sp (μm) | 108.4 (66.1–112.1) | 94.1 (74.8–104.9) | 112.9 (72.0–128.5) | 90.6 (54.4–128.1) | |
| Tb.N (mm−1) | 6.10 (5.37–7.78) | 6.68 (6.00–7.13) | 5.60 (5.19–7.70) | 6.72 (5.58–8.33) | |
| Diaphysis | Ct.Ar (mm2) | 4.30 (4.01–4.74) | 4.40 (3.97–4.77) | 4.22 (3.89–4.67) | 4.08 (3.62–4.76) |
| Ma.Ar (mm2) | 3.90 (3.19–4.73) | 3.97 (3.46–5.13) | 3.91 (3.59–5.19) | 3.95 (3.61–4.53) | |
| Ma.Ar/Tt.Ar | 0.476 (0.443–0.503) | 0.485 (0.442–0.553) | 0.485 (0.446–0.552) | 0.492 (0.441–0.509) | |
| Ps.MAR (µm/day) | 6.37 (3.17–8.02) | 5.11 (3.96–8.36) | 5.79 (5.15–6.60) | 6.02 (4.94–8.03) | |
| Es.MAR (µm/day) | 4.45 (3.15–5.69) | 3.73 (2.82–7.19) | 5.37 (3.14–6.20) | 4.56 (3.71–6.24) | |
| Epiphyseal cartilage | Reserve zone width (μm) | 38.1 (28.7–40.5) | 40.1 (34.0–41.5) | 39.3 (31.9–48.5) | 36.6 (30.5–41.6) |
| Proliferative zone width (μm) | 50.1 (45.1–53.2) | 48.8 (38.8–57.9) | 52.8 (41.7–55.5) | 44.8 (42.0–52.2) | |
| Hypertrophic zone width (μm) | 39.6 (33.9–45.0) | 42.0 (33.3–43.9) | 41.6 (37.4–47.3) | 37.0 (34.0–39.1) | |
Notes: Results are presented as median (min–max); n=8–10. Loratadine at doses of 0.5 mg/kg, 5 mg/kg, or 50 mg/kg was administered orally, once daily for 4 weeks. Kruskal–Wallis test followed by Mann–Whitney U test were used for statistical evaluation of loratadine effect in comparison with the control rats.
Abbreviations: BV/TV, bone volume/tissue volume ratio; C, control rats; Ct.Ar, transverse cross-sectional area of the cortical bone; Es.MAR, endosteal mineral apposition rate; L0.5, rats treated with loratadine at a dose of 0.5 mg/kg p.o.; L5, rats treated with loratadine at a dose of 5 mg/kg p.o.; L50, rats treated with loratadine at a dose of 50 mg/kg p.o.; Ma.Ar, transverse cross-sectional area of the marrow cavity; Ma.Ar/Tt.Ar, transverse cross-section of the marrow cavity/total diaphysis area ratio; Ps.MAR, periosteal mineral apposition rate; Tb.N, trabecular number; Tb.Sp, trabecular separation; Tb.Th, trabecular thickness.