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. 2019 Sep 23;11:8571–8575. doi: 10.2147/CMAR.S217362

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© 2019 Caselli et al.

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Individual cytokines altered by bacterial co-colture. Endometrial cells were cultured in the absence (control, CTR) or presence of alive or killed A. vaginae (AV), or P. somerae (PS), or L. vaginalis (LV), or a combination of A. vaginae plus P. somerae (AV+PS). Cell culture supernatants collected at 24 h.p.i. were analyzed for cytokine release by individual ELISA assay, performed for each cytokine resulted altered by the multi-cytokine ELISA panel. Results refer to triplicate samples from two independent experiments and are expressed as mean pg/mL value ± SD. Student’s t test was used for statistical analysis; a p value < 0.05 was considered as significant (indicated by asterisks).