Figure 3.
DCVacc/VSV‐GP combination treatment increased the numbers of tumor infiltrating lymphocytes (TIL). B16‐OVA melanoma in C57BL/6 mice were treated i.t./p.t. on Days 11 and 18 posttransplantation with PBS (control), VSV‐GP (6 × 107 PFU), DCVacc (2 × 105 OVA‐loaded CpG‐matured bmDCs) or DCVacc/VSV‐GP. Seven days after the second treatment cells from B16‐OVA tumors were isolated and TILs were analyzed by FACS. Total cell numbers per g tumor were calculated for CD45 positive immune cells (a), CD3 T cells (b), CD4 T cells (c), CD8 T cells (d) and activated CD43 positive CD8 T cells (e). Data represent results from two independent experiments. Data were analyzed by ANOVA followed by Tukey's multiple comparisons test (*p ≤ 0.05,**p ≤ 0.01,***p < 0.001).