Figure 5.
Elevated Tconv/Treg and CD8/Treg ratios in VSV‐GP treated mice. B16‐OVA melanoma in C57BL/6 mice were treated i.t./p.t. on Days 11 and 18 posttransplantation with PBS (control), VSV‐GP (6 × 107 PFU), DCVacc (2 × 105 OVA‐loaded CpG‐matured bmDCs) or DCVacc/VSV‐GP. Seven days after the second treatment cells from B16‐OVA tumors were isolated and CD4/FoxP3 positive cells were analyzed by FACS. Percentage and total cell numbers per g tumor were determined for CD4/FoxP3 double‐positive Tregs. Ratios for FoxP3 negative conventional CD4 T cells and Tregs (Tconv/Treg) as well as for CD8 T cells and Tregs (CD8/Treg) were also calculated. Data represent results from two independent experiments. Data were analyzed by ANOVA followed by Tukey's multiple comparisons test (*p ≤ 0.05,**p ≤ 0.01,***p < 0.001).