Skip to main content
. 2019 May 10;223(3):1547–1559. doi: 10.1111/nph.15846

Figure 2.

Figure 2

Constitutive overexpression of NbGPAT6a renders Nicotiana benthamiana leaves more resistant to infection by Phytophthora infestans. (a) Confirmation of NbGPAT6a‐GFP overexpression in transgenic N. benthamiana leaves, compared with control plants constitutively expressing GFP alone, quantified by quantitative reverse transcription polymerase chain reaction (qRT‐PCR). Error bars represent ± SE of the mean of three biological replicates. Significant differences are indicated as: *, P < 0.05; **, P < 0.01; ***, P < 0.001. Blue circles represent the average of three technical replicates. (b) Quantification of necrotic area present on leaves constitutively expressing NbGPAT6a‐GFP compared with the control expressing GFP alone following inoculation with P. infestans. Error bars represent ± SE of the mean of at least seven leaves from each transgenic line. Blue circles represent necrotic area on each leaf. (c) Representative images of leaves inoculated with P. infestans and imaged under blue light illumination using a yellow filter to record plastid red autofluorescence and to quantify necrotic area. Scale bar, 30 mm.