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. 2019 Apr 29;112(2):461–481. doi: 10.1111/mmi.14250

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© 2019 The Authors. Molecular Microbiology Published by John Wiley & Sons Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Examples of S1 nuclease protection assays of σ^E target genes, divided into three classes. A. Class I genes, having a single promoter that is completely dependent on σ^E. B. Class II genes, having a single promoter that is partially dependent on σ^E. (C) Class III genes, having multiple promoters, one of which is partially dependent on σ^E. RNA was prepared from S. coelicolor M600 (WT) and the sigE null mutant J2130 (ΔsigE) after 0, 30, 60 and 90 minutes treatment with 10 µg/ml vancomycin. In (C), p4 is the σ^E target promoter of the sco3194 gene.