Fig. 3.

Overexpression of miR-145 inhibited multidrug resistance and cell proliferation-related signaling by down-regulating AKT3. a The mRNA level of AKT3 was detected by qRT-PCR in EC109 and KYSE-30 cells transfected with sh-AKT3 and pcDNA3.1-AKT3. b The protein level of AKT3 and p-AKT was assessed by Western blotting in EC109 and KYSE-30 cells transfection with sh-AKT3 and pcDNA3.1-AKT3. c Quantification of relative protein level for Western blotting. d The multidrug resistance and cell proliferation-related protein level was detected by Western blotting in EC109 and KYSE-30 cells transfected with miR-145 mimics, sh-AKT3 or pcDNA3.1-AKT3. All the results were shown as mean ± SD (n = 3), which were three separate experiments performed in triplicate. *p < 0.05