Table 3.
Transcriptome data of known cytosolic acetyl-CoA generating pathways and the genes related to heterologous IA production in A. niger
| Locus tag | Enzyme | Old locus tag | Localization | AB1.13 WT | AB1.13 CAD | ||
|---|---|---|---|---|---|---|---|
| RPKM | RPKM | ||||||
| ATP-citrate lyase | |||||||
| ANI_1_76094 | ATP-citrate lyase subunit 1 (aclA) | An11g00510 | Cyto | 325.61 | 325.45 | 506.32 | 505.54 |
| ANI_1_78094 | ATP-citrate lyase subunit 2 (aclB) | An11g00530 | Cyto | 331.2 | 332.23 | 587.4 | 587.02 |
| CAT system | |||||||
| ANI_1_724074 | Carnitine acetyl transferase (facC) | An08g04990 | Cyto/Nucleus | 28.78 | 29.17 | 24.89 | 24.77 |
| ANI-1-192164 | Carnitine acetyl transferase (acuJ) | An18g01590 | Per/Mito | 45.97 | 47.7 | 38.2 | 38.08 |
| ANI_1_388034 | Carnitine/acyl carnitine carrier (acuH) | An03g03360 | Cyto/Mito | 31.08 | 30.93 | 29.01 | 28.95 |
| PAA pathway | |||||||
| ANI_1_936024 | Pyruvate decarboxylase (pdcA) | An02g06820 | Cyto | 5910.75 | 5915.54 | 304.97 | 306.49 |
| ANI_1_1024084 | Pyruvate decarboxylase (pdcB) | An09g01030 | Cyto | 38.82 | 38.64 | 47.95 | 48.26 |
| ANI_1_2276014 | Pyruvate decarboxylase | An01g01590 | Nucleus | 0.35 | 0.45 | 0.17 | 0.18 |
| ANI_1_796114 | Pyruvate decarboxylase | An13g03320 | Mito/Cyto | 0 | 0 | 0.03 | 0.03 |
| ANI_1_1024074 | Aldehyde dehydrogenase (aldA) | An08g07290 | Cyto | 143.48 | 142.19 | 178.07 | 177.72 |
| ANI_1_226174 | Aldehyde dehydrogenase | An10g00850 | Per | 1.59 | 1.35 | 1.69 | 2.21 |
| ANI_1_1748184 | Aldehyde dehydrogenase | An04g03400 | Cyto | 54.25 | 54.85 | 53.22 | 54.15 |
| ANI_1_924184 | Acetyl-CoA synthetase (acuA) | An04g05620 | Cyto | 139.46 | 141.21 | 96.66 | 96.09 |
| ANI_1_938144 | Acetyl-CoA hydrolase (ach1) | An16g07110 | Mito | 98.53 | 99.38 | 22.36 | 22.93 |
| ANI_1_878024 | Acetate kinase | An02g06420 | Cyto/Mito | 42.53 | 43.41 | 13.72 | 13.75 |
| Mitochondrial acetyl-CoA synthesis | |||||||
| ANI_1_1206064 | Pyruvate dehydrogenase E1 component subunit alpha (pda1) | An07g09530 | Mito | 263.81 | 264.72 | 205.40 | 207.09 |
| ANI_1_622094 | Pyruvate dehydrogenase E1 component subunit alpha | An11g04550 | Mito | 10.81 | 9.47 | 10.54 | 9.75 |
| ANI_1_12014 | Pyruvate dehydrogenase E1 component subunit beta | An01g00100 | Mito | 215.18 | 213.19 | 177.40 | 176.43 |
| ANI_1_274064 | Pyruvate dehydrogenase E2 component | An07g02180 | Mito | 299.81 | 301.04 | 286.65 | 284.54 |
| Citrate synthases | |||||||
| ANI_1_876084 | Citrate synthase (citA) | An09g06680 | Mito | 478.40 | 482.85 | 428.47 | 426.67 |
| ANI_1_1226134 | Methylcitrate synthase (mcsA) | An15g01920 | Mito | 51.49 | 51.55 | 25.69 | 24.11 |
| ANI_1_1474074 | Citrate synthase (citB) | An08g10920 | Cyto | 58.09 | 57.90 | 522.44 | 521.45 |
| ANI_1_2950014 | Citrate synthase (citC) | An01g09940 | Cyto | 3.86 | 3.52 | 0.91 | 0.96 |
| Aconitases | |||||||
| ANI_1_1410074 | Aconitate hydratase (aco1) | An08g10530 | Mito | 234.09 | 234.83 | 397.94 | 397.68 |
| ANI_1_470084 | Aconitate hydratase | An09g03870 | Mito | 64.88 | 64.41 | 26.01 | 26.07 |
| ANI_1_3018024 | Aconitate hydratase | An02g11040 | Cyto | 0.04 | 0.04 | 0.00 | 0.00 |
| ANI_1_1808144 | Aconitate hydratase | An16g05760 | Cyto | 0.85 | 0.85 | 0.98 | 0.99 |
| ANI_1_578044 | Aconitase | An05g02230 | Cyto | 6.29 | 6.38 | 14.13 | 14.28 |
| ANI_1_1802134 | Aconitase | An15g07730 | Cyto | 26.63 | 26.21 | 30.62 | 30.72 |
| Itaconate biosynthesis | |||||||
| cis-aconitate decarboxylase (cadA) | Cyto | 1.27 | 1.20 | 4287.14 | 4300.13 | ||