Skip to main content
. 2019 Aug 22;597(18):4757–4778. doi: 10.1113/JP278454

Figure 8. Exercise‐induced proteolytic signalling in the skeletal muscle of SMA‐like animals.

Figure 8

A, representative western blots of the phosphorylated form of Unc‐51‐like autophagy‐activating kinase 1 (pULK1), total ULK1, sequestosome‐1 (p62), as well as the cytosolic microtubule‐associated protein 1A/1B‐light chain 3 LC3 (i.e. LC3I) and membrane bound LC3 (i.e. LC3II) in QUAD muscles of Smn2B/+ SED, Smn2B/− SED, Smn2B/− 0 h and Smn2B/− 3 h animals. A Ponceau S stain, indicative of equal loading between samples, is also displayed below. Approximate molecular mass markers (kDa) are denoted at the right of the blots. B–D, graphical summaries of protein expression and activation status of ULK1 (B), as well as the levels of p62 (C) and the LC3II:LC3I ratio (D). E, summaries of ULK1, beclin‐1‐associated autophagy‐related key regulator (ATG14), BCL2/adenovirus E1B 19 kDa protein‐interacting protein 3 (BNIP3), p62, GABAA receptor‐associated protein‐like 1 (Gabrapl1) mRNA expression in TA muscles from all experimental groups. F, summaries of muscle RING finger‐1 (MuRF1), and muscle atrophy F‐Box (MAFbx) mRNA expression in TA muscles from mice in the four experimental groups. G, gene expression summaries of ATG14 and p62 mRNA expression in QUAD muscles of Smn2B/+ SED, Smn2B/− SED and Smn2B/− 3 h animals. H, gene expression summaries of MAFbx in QUAD muscles of Smn2B/+ SED, Smn2B/− SED and Smn2B/− 3 h mice. All data are expressed relative to Smn2B/+ SED. * P < 0.05 vs. Smn2B/+ SED; # P < 0.05 vs. Smn2B/− SED; n = 7.