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Angiogenic activation of ad-MVFs. Western blot analysis of the expression (pixel intensity × 10³) of VEGF, VEGFR-2, and MMP-2 within ad-MVFs, which were cultivated for 24 h in 4°C UW solution supplemented with vehicle (white bars, n = 4), IGF-1 (black bars, n = 4), or a combination of IGF-1 and IGFbp4 (gray bars, n = 4). The data were normalized to β-actin signals to correct unequal loading.
Mean ± SEM; *p < 0.05 vs vehicle.
