Table 2.
Diameter (µm), centerline RBC velocity (µm s−1), and wall shear rate (s−1) of individual microvessels within ad-MVF-seeded collagen–glycosaminoglycan scaffolds on day 3, 6, 10, and 14 after implantation into dorsal skinfold chambers, as assessed by intravital fluorescence microscopy.
| Day 3 | Day 6 | Day 10 | Day 14 | |
|---|---|---|---|---|
| Diameter (µm) | ||||
| Vehicle | 55.5 ± 9.8 | 21.2 ± 0.8 | 15.7 ± 0.8 | 12.9 ± 0.5 |
| IGF-1 | 32.1 ± 6.7 | 21.8 ± 1.5 | 12.5 ± 0.5*# | 10.4 ± 0.4*# |
| IGF-1 + IGFbp4 | 39.6 ± 4.7 | 24.1 ± 1.8 | 15.0 ± 0.6 | 13.2 ± 0.7 |
| Centerline RBC velocity (µm s−1) | ||||
| Vehicle | 25.0 ± 3.0 | 194.4 ± 28.1 | 284.9 ± 33.5 | 360.1 ± 21.9 |
| IGF-1 | 39.5 ± 9.8 | 185.1 ± 28.9 | 385.2 ± 30.3*# | 451.3 ± 13.2*# |
| IGF-1 + IGFbp4 | 13.6 ± 11.1 | 96.7 ± 16.6 | 243.0 ± 34.7 | 341.1 ± 23.4 |
| Wall shear rate (s−1) | ||||
| Vehicle | 5.0 ± 2.3 | 86.7 ± 12.3 | 178.8 ± 23.9 | 259.0 ± 17.0 |
| IGF-1 | 26.6 ± 14.4 | 77.7 ± 13.4 | 276.9 ± 17.4*# | 402.8 ± 21.2*# |
| IGF-1 + IGFbp4 | 2.9 ± 2.4 | 39.0 ± 7.7 | 165.1 ± 30.0 | 255.1 ± 24.5 |
Ad-MVF: adipose tissue–derived microvascular fragments; UW: University of Wisconsin; IGF-1: insulin-like growth factor 1; IGFbp4: insulin-like growth factor–binding protein 4; RBC: red blood cell; SEM: standard error of the mean.
The ad-MVFs were cultivated for 24 h in 4°C UW solution supplemented with vehicle (n = 8), IGF-1 (n = 8), or a combination of IGF-1 and IGFbp4 (n = 8).
Mean ± SEM; *p < 0.05 vs vehicle; #p < 0.05 vs IGF-1 + IGFbp4.