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. 2019 Sep 27;11:1758835919875324. doi: 10.1177/1758835919875324

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© The Author(s), 2019

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — The high CLDN4 level is correlated with poor prognosis and poor CCRT response in ESCC. (a) Representative immunohistochemistry images of ESCC specimens showing CLDN4 expression scores from 0 to 3. Score 0, 1, and 2 were classified to CLDN4 low expression and score 3 is defined to CLDN4 high expression. Overall survival (left-upper) and disease-free survival (left-lower) of 139 ESCC patients were stratified by CLDN4 expression level by Kaplan–Meier analysis. Overall survival (right-upper) and disease-free survival (right-lower) of the 54 patients with stage I/II ESCC was stratified with CLDN4 expression level by Kaplan–Meier analysis. (b) The diagram shows the procedure to select CCRT resistance cells. The KYSE170 cells were received cisplatin combined with irradiation for indicating times and the lethal cells were removed before harvest protein lysate. The CCRT resistance cells were examined the CLDN4 levels and stemness genes by immunoblotting. Quantified results of immunoblotting indicated the relative protein expression level at different time points. (c) Immunohistochemistry showed the CLDN4 levels in xenotransplanted mice with or without CCRT treatment. (d) The tissues from CCRT responder or nonresponder ESCC patients were used to examine the CLDN4 levels by immunohistochemistry. (e) The percentage of increased CLDN4 was used to evaluate in both CCRT poor and good responders.

CCRT, cisplatin/concurrent chemoradiation therapy; ESCC, esophageal squamous cell carcinoma