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. 2019 Sep 24;11:8699–8710. doi: 10.2147/CMAR.S208508

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© 2019 Shao et al.

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TUG1 negatively regulates expression of KLF4 via interacting with EZH2. (A) The correlation between TUG1 and KLF4 was analyzed by Spearman’s correlation analysis (r=−0.549, P<0.001). (B) The levels of KLF4 mRNA were detected by qPCR when SW480 and HCT116 cells transfected with si-TUG1 and results are expressed relative to the corresponding values for control cells. (C–D) The levels of KLF4 protein levels were determined by Western Blotting when SW480 and HCT116 cells were transfected with si-TUG1. (E) The levels of EZH2 protein were detected by Western Blotting when SW480 and HCT116 cells transfected with si-EZH2 and results are expressed relative to the corresponding values for control cells. (F) The levels of KLF4mRNA were detected by qPCR when SW480 and HCT116 cells transfected with si-EZH2 and results are expressed relative to the corresponding values for control cells. (G–H) The levels of KLF4 protein were detected by Western Blotting when SW480 and HCT116 cells transfected with si-EZH2 and results are expressed relative to the corresponding values for control cells. (I–J) TUG1 expression levels in cell cytoplasm or nucleus of HCC cell lines SW480 and HCT116 were detected by qPCR. (K–L) ChIP-qPCR analysis of IgG, EZH2 occupancy in the KLF4 promoter region with or without TUG1 silencing in SW480 and HCT116 cells. Values were expressed as mean ± SEM. ***P<0.001, **P<0.01.

Abbreviations: TUG1, taurine up-regulated 1; KLF, Kruppel-like factor 4.