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PEGASOS tissue clearing method rendered craniofacial bones transparent. (a) Brief description of the PEGASOS tissue clearing procedure. Images of calvarial bone (b1–b3), frontal bone (c1–c3), palate (d1–d3), and mandible (e1–e3) before and after clearing with the PEGASOS decalcification method or nondecalcification method. Scale bars, 2 mm. PEGASOS, Poly(Ethylene Glycol)Associated Solvent System.
