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. 2019 Sep 26;28(19):1310–1321. doi: 10.1089/scd.2019.0104

FIG. 2.

FIG. 2.

Osteogenesis of parietal bone is gradually restricted to the suture and bone marrow regions during postnatal development. Calvarial bones were collected from mice at age of P1, P7, P14, or P21. Calcein green injection was performed 2 h before sacrifice. Three-dimensional imaging or histological sectioning was performed. Green, calcein green signal. White, SHG signal from the bone. Blue, DAPI staining. (a1, a2) Dorsal (a1) and ventral views (a2) of the calvarial bone of P1 mouse pups. (a3–a5) Histological sections of P1 sagittal suture. Boxed regions in (a3) were enlarged in (a4, a5) respectively. (b1, b2) Dorsal (b1) and ventral views (b2) of calvarial bones of P7 mouse pups. (b3–b5) Histological sections of P7 sagittal suture. Boxed regions in (b3) were enlarged in (b4, b5) respectively. (c1, c2) Dorsal (c1) and ventral views (c2) of the sagittal suture of P14 mice. (c3) The optical section at the calvarial bone center region was acquired to reveal calcein green within the bone marrow space (arrowheads). (c4–c6) Histological sections of the sagittal suture of P14 mice. Boxed regions in (c4) were enlarged in (c5, c6), respectively. (d1, d2) Dorsal (d1) and ventral view (d2) of the sagittal suture of P21 mouse. (d3) The optical section was acquired to reveal calcein green within the calvarial bone marrow space (arrowheads). (d4–d6) Histological sections of P21 sagittal suture. Boxed regions in (d4) were enlarged in (d5, d6), respectively. Asterisks indicate the calcein green signal underneath the periosteum. Round dots indicate calcein green signal underneath the dura. Arrows indicate signal surrounding the sagittal suture. Dotted lines outline sagittal sutures. S.s, sagittal suture. Scale bars, 50 μm in (a4, a5, b4, b5, c5, c6, d5, d6); 100 μm in other panels. 3D, three-dimensional; SHG, Second Harmonic Generation.