FIG. 5.

Spatial association of osteogenesis with vasculature in calvarial bone during the first 2 weeks after birth. Calcein green injection was performed before sacrificing mice. Three-dimensional images were acquired with a two-photon microscope. (a) Distribution of vasculature (red) and calcein green signal (green) in the calvarial bone at the age of P7. The dotted line outlines calvarial bone. Boxed regions were enlarged in (a1, a2) as indicated. (b) Distribution of vasculature (red) and calcein green signal (green) in the calvarial bone at the age of P14. The dotted line outlines calvarial bone. Boxed regions were enlarged in (b1–b3) as indicated. Vascular density and osteogenic intensity at different locations were quantified in (c, d). *P < 0.0083. Cor. S, coronal suture; Lamb. S, lambdoid suture; n.s., no significant difference; Sag. S, sagittal suture. Scale bars, 100 μm.