FIG. 6.

Spatial association of vasculature with osteogenesis in mouse calvarial bone at P21. Calvarial bones were collected from Cdh5-Cre; Ai14 mice at P21. Calcein green injection was performed before sacrificing mice. Samples were cleared following the PEGASOS method without decalcification. (a) Maximum project view of the vasculature (red) and calcein green signal (green) in the calvarial bone. Boxed regions were enlarged in (b, b′, c, c′, d, d′ and e) as indicated. The dotted line outlines calvarial bone. Vascular densities and calcein green signal intensities at different locations on the calvarial bone were quantified in (f, g). *P < 0.0083. Cor. S, coronal suture; Lamb. S, lambdoid suture; Sag. S, sagittal suture. Scale bars, 250 μm.