Fig 1.

Timeline of animal Phen treatment study design. Mice were first evaluated for their baseline sensorimotor, motor coordination/balance, and motor asymmetry functions by adhesive removal test (ART), beam walking test (BWT), and elevated body swing test (EBST) 1 week before CCI injury (PRE). On day 0, mice were given CCI or sham procedures, and 30 min after injury, they received a first injection of Phen (2.5mg/kg body weight, i.p.) or saline. Eight hours after the first injection, a second injection was provided. All mice received two injections of Phen or saline for five consecutive days. Behavioral tests were performed on day 7 and 14 after CCI, and thereafter, mice were euthanized for assessment of contusion volume and histology and immunocytochemistry. A similar timeline was used for Posiphen treatment.