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. 2019 Jul 15;28(9-10):1132–1139. doi: 10.1177/0963689719863809

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© The Author(s) 2019

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Separation of SSEA-3⁺ cells from mouse adipose-MSCs. (A) Expression of MSC surface markers were measured by flow cytometry in MSCs derived from mouse adipose tissue. (B) Representative FACS results showing the SSEA-3⁺ cells present in mouse adipose-MSCs. (C) Representative flow cytometry analyses after MACS, showing SSEA-3⁺ cell percentages. (D) Examples of M-clusters formed from a Muse cell recovered after sorting by FACS or MACS. Scale bars = 100 μm. (E) The rates of cluster formation were similar between Muse cells sorted by FACS or MACS (independent-sample t-test, P > 0.05; n = 3/group). FACS, fluorescence-activated cell sorting; MACS, magnetic-activated cell sorting; MSC, mesenchymal stem cell; SSEA-3, stage-specific embryonic antigen-3.