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. 2019 Jul 15;28(9-10):1132–1139. doi: 10.1177/0963689719863809

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© The Author(s) 2019

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — Muse cell characteristics of SSEA-3⁺ cells sorted from mouse adipose-MSCs. (A) Self-renewal capacity of mouse adipose-Muse cells, as determined by staining for ALP activity in first-, second-, and third-generation M-clusters. Adipose-MSCs were negative for ALP activity. (B) Immunocytochemistry of clusters formed from Muse cells in single-cell suspension cultures, showing that the clusters were positive for Nanog, Oct3/4, Sox2, and SSEA-3. Nuclei were visualized by staining with Hoechst dye. (C) Muse cells in single-cell suspension culture formed clusters in which the cells differentiated spontaneously, expressing endodermal (α-FP), mesodermal (SMA), and ectodermal (NF) markers. Scale bars = 100 µm. α-FP, alpha-fetoprotein; ALP, alkaline phosphatase; M-cluster, Muse cell-derived cell cluster; NF, neurofilament; SMA, smooth muscle actin.