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. 2019 Jun 28;28(9-10):1271–1278. doi: 10.1177/0963689719860840

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© The Author(s) 2019

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 2. — Exosome genes are upregulated in MetS-EVs. (A) Statistically significant exosome genes with a fold-change ≥1.4, compared with Lean EV. (B) Heat map depicting the differentially expressed exosome genes in Lean- vs. MetS-EVs. (C) Lysosomal activity assay performed using imaging flow cytometer, showing the intensity of intracellular uptake of the self-quenched substrate and percentage of positively stained cells. (D) Western blot analysis showed increased protein expression of RhoF in MetS-MSC. **p ≤ 0.01, (vs. Lean); BF, Brightfield; SQS, Self-quenched substrate; SSC, side scatter.