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. 2019 Jul 4;28(9-10):1242–1256. doi: 10.1177/0963689719857657

Figure 2.

Figure 2.

Localization of collagen VII (COL7) in sections of engineered skin substitutes (ESS) in vitro. Shown are sections of ESS prepared with RDEB fibroblasts and RDEB keratinocytes (group 1; A-D), RDEB fibroblasts and normal keratinocytes (group 2; E–H), normal fibroblasts and RDEB keratinocytes (group 3; I–L), and normal fibroblasts and normal keratinocytes (group 4; M–P). Note that each row depicts a single section photographed using different fluorescent illumination. Immunohistochemistry was performed to localize COL7 using two different antibodies: a monoclonal antibody (B, F, J, N; red), specific for human COL7, and a polyclonal antibody (C, G, K, O; green) that cross-reacts with mouse COL7. Nuclei were counterstained using 4′,6-diamidino-2-phenylindole (DAPI; A, E, I, M; blue). Arrows in A, E, I, and M indicate location of dermal–epidermal junction. Scale bar in A (100 µm) is same for all panels.