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. 2019 Jul 4;28(9-10):1242–1256. doi: 10.1177/0963689719857657

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© The Author(s) 2019

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 6. — Localization of collagen VII (COL7) in engineered skin substitutes (ESS) in vivo. Immunohistochemistry was performed to localize COL7 using two different antibodies: a monoclonal antibody (red), specific for human COL7, and a polyclonal antibody (green) that cross-reacts with mouse COL7. Nuclei were counterstained using DAPI (blue). Merged fluorescent images are shown. Shown are sections of ESS from week 1 (left; A, D, G, J), week 2 (center; B, E, H, K), and week 3 (right; C, F, I, L) in vivo. ESS were prepared with RDEB fibroblasts and keratinocytes (group 1; A–C), RDEB fibroblasts and normal keratinocytes (group 2; D–F), normal fibroblasts and RDEB keratinocytes (group 3; G–I), and normal fibroblasts and keratinocytes (group 4; J–L), as indicated. Arrows (A–C) indicate locations of dermal–epidermal junctions, and asterisks (C) indicate epidermal blistering, in ESS prepared with all RDEB cells. Scale bar in A (50 µm) is same for all panels.