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. 2019 Jul 4;28(9-10):1299–1305. doi: 10.1177/0963689719861063

Fig. 3.

Fig. 3.

FSCN1 is the direct target of miR-145. (A) Relative luciferase activity of HeLa cells after co-transfection with wild-type (WT) or mutant FSCN1 3’ UTR reporter genes and miR-145 or miR-Ctrl. The relative luciferase activity was normalized to Renilla activities. *p < 0.05 vs miR-Ctrl. (B, C) miR-145 and FSCN1 expression levels after miR-145 overexpression were detected by qRT-PCR. (D) The FSCN1 protein levels were analyzed by Western blotting in cells overexpressing miR-Ctrl and miR-145 mimics. The results are presented as mean ± SE (n = 3). *p < 0.05 vs mock cells; **p < 0.05 vs miR-Ctrl.