Figure 2.

Cytotoxicity increase and high mobility group box-1 release induced by H₂O₂ in BNL CL cells. A and B: BNL CL2 cells cultured with H₂O₂ for 0, 0.5, 2, 8, or 24 h or at different concentrations (0, 100, 500, and 1000 μmol/L); C: 8-hydroxy-2-deoxyguanosine levels in cells treated with 500 μmol/L H₂O₂ or N-acetylcysteine (NAC); D: High mobility group box-1 (HMGB1) content in medium detected by ELISA; E: Cells were treated with 500 μM H₂O₂ for different time intervals HMGB1 content in medium detected by ELISA. Cells were pre-treated with NAC for 24 h, and then incubated with or without H₂O₂ (500 μmol/L) for 8 h. All data are presented as the mean ± SD. Statistical analysis was done by the Student’s t-test. ^aP < 0.05 vs 0 group, ^bP < 0.01 vs 0 group, ^dP < 0.01 vs H₂O₂ group. 8-HOdG: 8-hydroxy-2-deoxyguanosine; NAC: N-acetylcysteine; HMGB1: High mobility group box-1.