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Locations and sequences of the primers on the P1 gene. Location and sequence of the P1 gene (M. pneumoniae-specific gene) used to design multiple cross displacement amplification (MCDA) primers. The locations of the P1 gene of M. pneumoniae was from 180,858 to 185,741. The nucleotide sequence of the sense strand of the P1 gene is shown. Right arrows and left arrows indicate sense and complementary sequences that were used.
