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Effects of the HT extract on the relevant proteins in the LPS-induced RAW264.7 cells. Cells were pre-treated with the extract at the concentrations of 100, 200, 400 μg/mL in the 6-well plates for 1 h prior to the stimulation with LPS (1 μg/mL) for 12 h or 1 h, and the relevant proteins deduced from the computational predictions were analyzed by western blotting (A, C and D) and immunofluorescence staining (B) subsequently.
