Figure 1.

Strain-inspired and cannabinoid or terpene mixtures initiate calcium responses in a TRPV1 over-expression system. a. HEK-TRPV1 differentiate TRPV1-dependent calcium responses. HEK WT and HEK-TRPV1 were loaded with Fluo-4 and population-based calcium assays were conducted in the presence of 1mM external calcium. After a non-stimulated (NS) period to establish a baseline, cells were stimulated at 20 s with 100 nM capsaicin. b, c, d. HEK-TRPV1 were exposed to a matched vehicle mixture (veh) or the indicated mixtures of cannabinoids plus terpenes, cannabinoids, or terpenes, as indicated. Fluo-4 calcium assays were performed as described. e. Distribution of content values of terpenoids in 2,662 Cannabis samples representing 396 “strains” in three major chemovars [41,42]. The box plots display the range and distribution of each analyte in terms of %w/w. The line bisecting each box represents the median for that distribution. The lower and upper lines show the minimum and maximum values of the lower and upper quartiles, respectfully. The points show the outliers for the sampled ranges. The y-axis values represent % (w/w) of dried flower for each of the indicated cannabinoid species.