Figure 3.

Dependency of terpene and myrcene responses on presence of TRPV1. a–c. HEK WT and HEK-TRPV1 were loaded with Fluo-4 and population-based calcium assays were conducted in the presence of 1mM external calcium. After a non-stimulated (NS) period to establish a baseline, cells were stimulated at 20 s with the indicated compound or mixture. d-g. Myrcene dose-response. Area under the curve analyses were performed followed by Student’s t-test. P value for HEK TRPV1 relative to HEK WT: panels A–G, P < 0.005. h, i. Effect of TRPV1 antagonist Capsazepine on calcium responses initiated by Myrcene in HEK-TRPV1 cells. Area under the curve analyses were performed followed by Student’s t-test. P value for trace in panel H relative to I: P = 0.002. j. Comparison of Myrcene induced (two doses, left and right panels) calcium responses in HEK TRPV1 stimulated in the absence (red trace, 0 mM added CaCl₂ plus 1 mM EGTA) or presence of external calcium. Area under the curve analyses were performed followed by Student’s t-test. P-value for 1mM relative to 0mM external Ca²⁺: left panel, P = 0.018; right panel, P = 0.0008.