Figure 8.

Myrcene suppression of subsequent TRPV1 ligand responses.a–d. CBD initiation of TRPV1 conductances in HEK TRPV1.Cannabidiol (CBD), Cannabividarin, Can nabigerolic Acid, Cannabichromene and Cannabidiolic Acid were found to be effective TRPV1 ligands both in bulk Ca²⁺ assay and using electrophysiology [46]. a. CBD induction of outwardly rectifying conductance in HEK TRPV1. b. CBD-induced current is sensitive to application of the TRPV1 inhibitor Capsazepine. c. CBD-induced current is sensitive to washout. d. Current-voltage relationship for CBD-induced TRPV1.e. Fluo-4 Ca²⁺ responses in HEK TRPV1 stimulated according to the schema shown in Table I. All concentrations are in micromolar. For all three protocols, Myrcene was allowed to occupy TRPV1 in the absence of external Ca²⁺ (i). For the second recording phase (ii), 1 mM external Ca²⁺ was restored or Ca²⁺-free conditions were maintained and cannabidiol or myrcene were applied. For the third phase (iii), Ca²⁺ restoration and addition of stimuli were performed for those samples that saw Ca²⁺-free conditions in phase 2.