Table 1. Sequences of primers used for qRT-PCR.
Gene name | Sequence (5′–3′) |
---|---|
FoxO1-F1 | AGTGGATGGTGAAGAGCGTG |
FoxO1-R1 | GAAGGGACAGATTGTGGCGA |
Fasn-F1 | CAAGTGTCCACCAACAAGCG |
Fasn-R1 | GGAGCGCAGGATAGACTCAC |
Pepck-F1 | TGCGGATCATGACTCGGATG |
Pepck-R1 | AGGCCCAGTTGTTGACCAAA |
Scd1-F1 | GGCTAGCTATCTCTGCGCTC |
Scd1-R1 | GAACTGCGCTTGGAAACCTG |
HMGCoAR-F1 | ACGATCCTTCCTTATTGGCGG |
HMGCoAR-R1 | CTCCGGATCTCAATGGAGGC |
Fatp1-F1 | GGCAAGCTCCAGCACAGGAT |
Fatp1-R1 | ACCCACGTACACACAGAACG |
G6Pase-F1 | CGACTCGCTATCTCCAAGTGA |
G6Pase-R1 | GTTGAACCAGTCTCCGACCA |
SREBP1c-F1 | GATGTGCGAACTGGACACAG |
SREBP1c-R1 | CATAGGGGGCGTCAAACAG |
Cyp7a1-F1 | GGGATTGCTGTGGTAGTGAGC |
Cyp7a1-R1 | GGTATGGAATCAACCCGTTGTC |
Abcg1-F1 | CTTTCCTACTCTGTACCCGAGG |
Abcg1-R1 | CGGGGCATTCCATTGATAAGG |
Fabp1-F1 | ATGAACTTCTCCGGCAAGTACC |
Fabp1-R1 | CTGACACCCCCTTGATGTCC |
Cd36-F1 | ATGGGCTGTGATCGGAACTG |
Cd36-R1 | GTCTTCCCAATAAGCATGTCTCC |
Acc1-F1 | ATGGGCGGAATGGTCTCTTTC |
Acc1-R1 | TGGGGACCTTGTCTTCATCAT |
PPARα-F1 | AGAGCCCCATCTGTCCTCTC |
PPARα-R1 | ACTGGTAGTCTGCAAAACCAAA |
PPARγ-F1 | TCGCTGATGCACTGCCTATG |
PPARγ-R1 | GAGAGGTCCACAGAGCTGATT |
Pdk4-F1 | AGGGAGGTCGAGCTGTTCTC |
Pdk4-R1 | GGAGTGTTCACTAAGCGGTCA |
Cpt1a-F1 | CTCCGCCTGAGCCATGAAG |
Cpt1a-R1 | CACCAGTGATGATGCCATTCT |
Acox1-F1 | TAACTTCCTCACTCGAAGCCA |
Acox1-R1 | AGTTCCATGACCCATCTCTGTC |
Lcad-F1 | TCTTTTCCTCGGAGCATGACA |
Lcad-R1 | GACCTCTCTACTCACTTCTCCAG |
Mcad-F1 | AGGGTTTAGTTTTGAGTTGACGG |
Mcad-R1 | CCCCGCTTTTGTCATATTCCG |
Ucp2-F1 | TGCACTCCTGTGTTCTCCTG |
Ucp2-R1 | GGGACCTTCAATCGGCAAGA |
β-actin-F1 | TTCTTGGGTATGGAATCCTGT |
β-actin-R1 | AGCACTGTGTTGGCATAGAG |