Table 3.
The biomarkers which worked for diagnostic of CRC in Exosome
| Biomarker | Technique of exosome isolation | Technique of exosome validation | Technique of markers detection or validation | Related marker | Patients (number/type) | Patient stage | Author/year: | PMID |
|---|---|---|---|---|---|---|---|---|
| KRTAP5-4, MAGEA3 | Centrifugation syringe filter | TEM, NTA, light microscope | Bioinformatic Analysis, RT-PCR | lncRNA | 30 patients, 30 control/Blood | I–IV | Dong, 2016.79 | 27,197,301 |
| GPC1 | ExoCapTM | TEM, Flow cytometry, Western blotting | Flow cytometry, Western blot analysis | miR‐96‐5p, miR‐149, miR‐182‐5p | 102 patients, 89 control/tissue and Blood, Cell line (HT‐29 & HCT‐116), Mouse | I–II | Li, 2017.78 | 28,233,416 |
| EPCAM | PEG | ELISA, SEM | qRT-PCR, SEM, DLS, ELISA | – | HCT‐116 Cell line | – | Manri, 2016.77 | 27,917,441 |
| UBC, H3F3A, HIST2H2AA3, AKT3, HSPA1B | GSE100206, GSE100063, GSE32323 (Bioinformatic Analysis) | – | 29 patients, 49 control/tissue and Blood | – | Huang, 2018.81 | doi: 10.21037/tcr.2018.05.32 | ||
| OATP1B3 | Exosome Isolation kit (Thermo Fisher Scientific), PVDF filter and Differential centrifugation | TEM, Western blotting | qRT-PCR, Western blotting | – | HCT116, HT-29, and SW480 cell line, Blood of Mouse | – | Morio, 2018.80 | 29,491,222 |
Abbreviations: TEM, transmission electron microscopy; NTA, nanoparticle tracking analyzer; PEG, polyethylene glycol polymer; ELISA, enzyme-linked immunosorbent assay; SEM, scanning electron microscope; DLS, dynamic light-scattering.