Skip to main content
. 2019 Sep 10;10(1):783–792. doi: 10.1080/21505594.2019.1661721

Figure 2.

Figure 2.

Adherence of ΔovrB mutants in vitro. (a,b) Adherence of O157 WT, ΔovrB mutant, and ovrB complementary strain to HeLa (a) and Caco-2 (b) cells. (c) Growth of O157 WT, ΔovrB mutant, and ovrB complementary strain in LB medium. (d) Detection of AE lesion formation by O157 WT, ΔovrB mutant, and ovrB complementary strain by FAS in HeLa cells at 3 h. The HeLa cell actin cytoskeleton (green) and nuclei of bacterial and HeLa cells (red) are shown. AE lesions are indicated by arrowheads. (e) The number of pedestals/infected HeLa cells by O157 WT, ΔovrB mutant, and ovrB complementary strain (n = 150 cells). (f) qRT-PCR analysis of changes in LEE gene expression in O157 WT, ΔovrB mutant, and the ovrB complementary strain. Data represent mean ± SD (n = 3). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 (Student’s t-test).