Figure 2.

Suppression of PIN7 expression by JA.

(a) Images showing the distribution and intensity of GFP signals in ARR5::GFP transgenic plants grown in JA-untreated (Con) and -treated conditions (JA) for 5 days (JA, 10 μM of methyl jasmonate). (b) Expression levels of PIN3 and PIN7 genes in the ARR5::GFP transgenic plants. Data represent mean values of three technical replicates, and error bars indicate SD. Asterisks show statistically significant differences between JA-untreated and -treated samples (p-value < 0.01, Student’s t-test). GAPDH (At1G13440) was used as an internal control to normalize gene expression. (c) Semi-quantitative RT-PCR results showing that expression levels of PIN3 and PIN7 in wild-type plants (Col-0) grown in JA-untreated and -treated conditions (10 μM of methyl jasmonate) for 9 days. ACT2 (At3G18780) was used as an internal control. Scale bar = 20 μm.