Table 2.
PCR primer-pairs used in qPCR.
| S. No. | Gene | Primer pair |
|---|---|---|
| 1 | ISG15 | Forward, 5ʹ- GAGGCAGCGACTCATCTTT-3ʹ and Reverse, 5ʹ-AGCATCTTCACCGTCAGGTC-3’ |
| 2 | VWA1 | Forward, 5ʹ-TCTCGCTAGGATGCTTTACTCC-3ʹ and Reverse, 5ʹ-CGTTCACAAGCTGTCCTGTC-3’ |
| 3 | SOS1 | Forward, 5ʹ-CAAGTTCCCCCTAATTTGACAT-3ʹ and Reverse, 5ʹ-CAACCTCCTCCCCCATAATAA-3’ |
| 4 | SLC33A1 | Forward, 5ʹ-CCTTGAATCTGCCGACTTTT-3ʹ and Reverse, 5ʹ-TGTTATTAAAAATACAGTTCCCCAGA-3’ |
| 5 | MUSTN1 | Forward, 5ʹ-GGCTCCACTCAGATCTTTTCC-3ʹ and Reverse, 5ʹ-ATAGGGGCTTCCTGAGCAC-3’ |
| 6 | ZNF638 | Forward, 5ʹ-CCAAGGTGATCTCACACTCCT-3ʹ and Reverse, 5ʹ-AAACCATGTTCTTTGTTTGTTTAAGA-3’ |
| 7 | CTIF | Forward, 5ʹ-GCGAAGGCTAAAGGAAAAGG-3ʹ and Reverse, 5ʹ-TCAGGATCTCGATCAGCTTG-3’ |
| 8 | ACTB (housekeeping gene) | Forward, 5ʹ-CCAACCGCGAGAAGATGA-3ʹ and Reverse, 5ʹ-CCAGAGGCGTACAGGGATAG-3’ |
| 9 | G6PD (housekeeping gene) | Forward, 5ʹ-AACAGAGTGAGCCCTTCTTCA-3ʹ and Reverse, 5ʹ-GGAGGCTGCATCATCGTACT-3ʹ. |