Table 1.
parameter | units | SC WAT | MES WAT | EPI WAT | p |
---|---|---|---|---|---|
cell counts | |||||
adipocyte | cellsx106/g WAT | 3.19 ± 0.84 | 4.59 ± 0.98 | 2.90 ± 0.35 | NS |
nucleated stromal cells | cellsx106/g WAT | 43.8 ± 7.7 | 59.8 ± 3.8 | 27.9 ± 7.3 | 0.0220 |
red blood cells | cellsx106/g WAT | 108 ± 51 | 236 ± 57 | 150 ± 61 | NS |
cell fragments | 106/g WAT | 151 ± 50 | 103 ± 22 | 105 ± 18 | NS |
WAT adipocytes recovery | % | 70.4 ± 7.6 | 83.0 ± 8.6 | 77.2 ± 0.1 | – |
cell volumes | |||||
adipocyte volume | pL/cell | 251 ± 30 | 159 ± 33 | 309 ± 7 | 0.0065 |
nucleated stromal cells | fL/cell | 419 ± 28 | 255 ± 79 | 256 ± 44 | NS |
red blood cells | fL/cell | 32.4 ± 1.9 | 32.4 ± 1.9 | 32.4 ± 1.9 | – |
red blood cells | % stromal cells | 62.8 ± 12.3 | 79.4 ± 3.3 | 84.4 ± 2.9 | NS |
cell fragments and droplets | fL/fragment | <5 | – | ||
other tissue data | |||||
debris (dry weight) | mg/g WAT | 37 | 59 | 22 | – |
water in intact tissue | mg/g WAT | 220 ± 63 | 160 ± 37 | 64.4 ± 11.2 | NS |
water in tissue minus debris | mg/g WAT | 213 ± 62 | 152 ± 41 | 62.9 ± 10.2 | NS |
fat in intact tissue | mg/g WAT | 740 ± 20 | 744 ± 24 | 871 ± 12 | 0.0014 |
fat in tissue minus debris | mg/g WAT | 713 ± 29 | 701 ± 62 | 852 ± 1 | 0.0440 |
tissue density | g/g WAT | 0.9246 ± 0.0130 | 0.9208 ± 0.0129 | 0.9282 ± 0.0048 | NS |
fat density | g/mL WAT | 0.922 ± 0.022 | – | ||
debris + interstitial space | µl/g WAT | 237 ± 97 | 275 ± 92 | 145 ± 106 | – |
The data are the mean ± SD of different [N: PG = 2; MES = 3; SC = 4] 2-rat tissue pools. Statistical significance of the differences between groups (1-way anova). The column p represents the p values corresponding to the effect of WAT site. NS = p > 0.05