Table 3.

WAT site expression (in specific mRNA copies per cell) of genes related with glycolysis and glycerol metabolism of young male Wistar rats.

gene		cells	SC WAT		MES WAT		EPI WAT		p
			7 mM	14mM	7 mM	14mM	7 mM	14mM	site	glucose
Glut1	glucose transporter 1	adipocytes	75.7 ± 5.0	128 ± 14*	304 ± 56*	287 ± 61	111 ± 11	123 ± 22	<0.0001	NS
		NCS	20.0 ± 4.8	26.3 ± 9.2*	31.1 ± 4.3*	52.6 ± 5.6*	16.3 ± 5.6*	23.3 ± 5.5	0.0050	0.0322
Hk1	hexokinase 1	adipocytes	172 ± 15	222 ± 29*	488 ± 52*	500 ± 35*	343 ± 54	394 ± 108	0.0011	NS
		NCS	11.9 ± 2.6	6.71 ± 4.04	26.0 ± 1.6	32.4 ± 0.1*	18.8 ± 6.7	20.5 ± 4.7	0.0007	NS
Ldha	type a lactate dehydrogenase	adipocytes	1003 ± 196	1274 ± 202*	2271 ± 260*	1963 ± 214	1959 ± 157	1988 ± 154	0.0003	NS
		NCS	95.6 ± 11.6	77.8 ± 11.8*	222 ± 9*	265 ± 38*	189 ± 20	168 ± 13	<0.0001	NS
Ldhb	type b lactate dehydrogenase	adipocytes	164 ± 21	176 ± 8*	332 ± 62*	362 ± 51*	339 ± 32	340 ± 23	0.0003	NS
		NCS	1.15 ± 0.18	0.66 ± 0.37*	3.63 ± 0.48*	1.96 ± 0.69*	3.94 ± 0.48	3.66 ± 0.32	<0.0001	0.0345
Pfkl	phospho-fructo-kinase liver	adipocytes	131 ± 11	195 ± 24	392 ± 73*	404 ± 24*	170 ± 29	182 ± 46	<0.0001	NS
		NCS	15.1 ± 2.6	7.56 ± 3.24	34.1 ± 1.2	41.2 ± 7.3*	17.2 ± 4.8	16.9 ± 3.3	<0.0001	NS
Phgdh	3-phospho-glycerate DH	adipocytes	17.5 ± 1.5	25.5 ± 3.7*	52.1 ± 11.6*	58.0 ± 9.8*	42.3 ± 4.7	44.0 ± 9.2	0.0013	NS
		NCS	2,64 ± 0.33	2.44 ± 0.53*	7.23 ± 0.96	8.14 ± 0.81*	5.40 ± 1.46	5.67 ± 1.60	0.0013	NS
Gpam	glycerol-3P acyl-transferase	adipocytes	15.6 ± 1.1	19.3 ± 4.7*	58.3 ± 10.7*	54.7 ± 15.6	38.8 ± 2.4*	34.7 ± 10.7*	0.0018	NS
		NCS	0.478 ± 0.100	0.439 ± 0.115*	1.12 ± 0.25	1.32 ± 0.54*	0.982 ± 0.137	0.904 ± 0.136	0.0298	NS
Pdk4	pyruvate DH kinase 4	adipocytes	4.76 ± 2.36	5.16 ± 2.52*	19.9 ± 15.9*	9.50 ± 1.81*	2.83 ± 0.92	2.72 ± 0.85	NS	NS
		NCS	0.061 ± 0.007*	0.024 ± 0.024*	0.050 ± 0.016	0.047 ± 0.002*	0.063 ± 0.009*	0.013 ± 0.003*	NS	NS
Fas	fatty acid synthase	adipocytes	97.2 ± 6.3	191 ± 30*	302 ± 54*	478 ± 84*	181 ± 27	241 ± 63	0.0004	0.0166
		NCS	5.42 ± 0.83	3.49 ± 0.99*	11.2 ± 1.8	19.7 ± 5.1*	8.62 ± 1.35	10.4 ± 1.9	0.0012	NS
Hsl	hormone-sensitive lipase	adipocytes	45.0 ± 5.5	38.4 ± 5.5*	48.6 ± 8.6*	48.4 ± 6.5	98.3 ± 34.1	109 ± 54	NS	NS
		NCS	0.739 ± 0.282	0.364 ± 0.079	0.966 ± 0.182	1.01 ± 0.15*	0.566 ± 0.050*	0.599 ± 0.011	0.0195	NS
Atgl	adipose TAG lipase	adipocytes	218 ± 20	320 ± 45	488 ± 65*	560 ± 56*	547 ± 71	465 ± 74*	0.0005	NS
		NCS	5.69 ± 0.85	5.22 ± 0.67*	11.8 ± 2.8	18.4 ± 1.03*	6.08 ± 1.48	6.08 ± 0.92	<0.0001	NS
Lpl	lipoprotein lipase	adipocytes	859 ± 42	1106 ± 228*	1437 ± 222*	1537 ± 238*	1466 ± 262	1681 ± 358	NS	NS
		stromal	2.62 ± 0.87	0.89 ± 0.20*	3.50 ± 0.38	3.51 ± 1.37*	3.32 ± 0.42	3.53 ± 0.26	0.0412	NS

The data are the mean ± sem of N = 4 different (* N = 3) two- rat pooled samples. Data in italics were obtained with 30 or more cycles. Statistical significance of the differences between groups (2-way ANOVA). The far right columns show the p values for site and initial glucose concentration. NS = p > 0.05