Figure 6.

Propranolol ameliorates tumor exosomes-associated cachectic wasting in vitro. The adipocytes or C2C12 in 50 μg of exosomes purified from cancer-associated conditioned medium (CA-CM) and/or Propranolol (PRO, 10 μM). (A) Mature adipocytes in the presence or absence of exosomes and/or Propranolol were stained with red oil (enlarged 100X). (B) Adipocytes were cocultivated in the presence or absence of exosomes and/or Propranolol. After 3 days, proteins were extracted for western blot analysis of the expression of the indicated proteins. (C) Immunofluorescence staining for myosin heavy chain 1 (MYH1) after treatment 24 h. Scale bars represent 50 μm. (D) After C2C12 cells were treated for 1 days, and Western blot analysis of related protein expression in different groups. The numbers represent the relatively quantitative results compared to the control group. Data are presented as the mean ± S.D. of at least three independent experiments. * P < 0.05 versus control values, # P < 0.05 versus control values as positive group.