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. 2019 Mar 23;8(1):83–97. doi: 10.1080/21623945.2019.1580842

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Dose-response of hepatocytes AML12 to oleic acid (OA) after 24 h. (a) Real-time analysis of OA uptake by enterocytes AML12 (left panel) and corresponding slopes and IC50 (right panel). Data are presented as mean values ± SEM (8 replicates). (b) OA-induced TG accumulation monitored by cytometry analysis of AdipoRed fluorescence. Mean AdipoRed-H was analyzed on living cell populations in duplicates (right panel). (c) Merged images of AdipoRed (green) and Hoechst 33258 (red) acquired with identical parameters with objective x20. Significant Student t-test-p-values are reported for p < 0.05, compared to control.