Figure 4.

Titration of ASCs number displaying successful differentiation capacity in feeder culture. (a and b) Increasing number of ASCs 1000, 5000, 10,000 and 50,000 were seeded on HFF layer. At d 14 post differentiation, cells were stained with Oil-Red-O and imaged with Nikon camera for whole well (a) or with a light microscope at 200× magnification for details. In (a)and (b), two biological repeats were used each. (c) Increasing number of ASCs 1000, 5000, 10,000 and 50,000 were cultivated independently in pure culture on plastic cell culture dishes. At d 14 post differentiation, cells were stained with Oil-Red-O and imaged with light microscope at 100× magnification. N = 3 donors; representative images from one donor are shown.