Skip to main content
. 2018 Sep 29;8(1):16–25. doi: 10.1080/21623945.2018.1521229

Figure 1.

Figure 1.

Age dependent altered reaction of adipose tissue to overnutrition. (A) Experimental scheme for feeding and treatment strategy. Different age groups of male mice (young (8 weeks), adult (16 weeks), middle aged (28 weeks), old (51 weeks) and very old (83 weeks)) were fed 4 days with HFD or chow diet in combination with EdU treatment to label proliferating preadipocytes in eWAT. (B-C) Intraperitoneal glucose tolerance test in (B) young (8 week) (chow n = 7; HFD n = 8) and (C) old (51 week) mice (chow n = 6; HFD n = 7). (D) Frequency distribution of adipocyte area and (E) mean adipocyte area in eWAT (8 weeks n = 5; 16 weeks n = 6; 28 weeks (chow n = 5; HFD n = 6); 51 weeks n = 4; 83 weeks n = 3; per group). (F) Quantification of EdU incorporation in preadipocytes (Lin-PDGFRa+) in eWAT (8 weeks n = 6; 16 weeks (chow n = 8; HFD n = 7); 28 weeks n = 6; 51 weeks (chow n = 7; HFD n = 9); 83 weeks n = 6). Error bars represent mean ± s.e.m. *(P < 0.05), **(P < 0.01), ***(P < 0.001), **** (P < 0.0001), by Student`s t-test (B-C and E-F), 2-way ANOVA + Bonferroni post hoc analysis (D). If not indicated otherwise n denotes individual mice. eWAT, epididymal adipose tissue; HFD, high fat diet; EdU, 5-Ethynyl-2ʹ-deoxyuridine.