Skip to main content
. 2018 Sep 29;8(1):16–25. doi: 10.1080/21623945.2018.1521229

Figure 3.

Figure 3.

Reaction of epididymal adipose tissue to a second short term HFD feeding. (A) Experimental scheme for feeding and treatment strategy. Mice were fed HFD for 4 days followed by a chow diet (intermediate feeding) for 2 or 7 weeks. This was followed by a second dietary intervention (refeeding) with chow (HCC) or HFD (HCH) in combination with EdU treatment for 4 days. (B-E) Intraperitoneal glucose tolerance test and corresponding AUC (2 weeks (HCC n = 7; HCH n = 8); 7 weeks (n = 8 per group). (F-I) Frequency distribution of adipocyte area and mean adipocyte area in eWAT after refeeding (2 weeks (HCC n = 4; HCH n = 3); 7 weeks (n = 5 per group)). (J-K) Quantification of EdU incorporation in preadipocytes (Lin-PDGFRa+) in eWAT after refeeding (2 weeks (n = 7 per group); 7 weeks (HCC n = 8; HCH n = 7). Error bars represent mean ± s.e.m. *(P < 0.05), **(P < 0.01), ***(P < 0.001), **** (P < 0.0001), by Student`s t-test (B-E, G, I, and J-K), 2-way ANOVA + Bonferroni post hoc analysis (F and H). Unless indicated otherwise, n denotes individual mice. eWAT, epididymal adipose tissue; HFD, high fat diet; EdU, 5-Ethynyl-2ʹ-deoxyuridine; AUC, area under curve.